Buccal DNA Collection: Comparison of Buccal Swabs with FTA Cards
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چکیده
منابع مشابه
Buccal DNA collection: comparison of buccal swabs with FTA cards.
Collection and analysis of DNA, most commonly from blood or buccal cells, is becoming more common in epidemiologic studies. Buccal samples, which are painless to take and relatively easily collected, are often the preferred source. There are several buccal cell collection methods: swabs, brushes, mouthwash, and treated cards, such as FTA or IsoCode cards. Few studies have systematically compare...
متن کاملShort Communication Buccal DNA Collection: Comparison of Buccal Swabs with FTA Cards
Collection and analysis of DNA, most commonly from blood or buccal cells, is becoming more common in epidemiologic studies. Buccal samples, which are painless to take and relatively easily collected, are often the preferred source. There are several buccal cell collection methods: swabs, brushes, mouthwash, and treated cards, such as FTA or IsoCode cards. Few studies have systematically compare...
متن کاملCollection of buccal cell DNA using treated cards.
We devised a simple, noninvasive, cost-efficient technique for collecting buccal cell DNA for molecular epidemiology studies. Subjects (n = 52) brushed their oral mucosa and expectorated the fluid in their mouths, which was applied to "Guthrie" cards pretreated to retard bacterial growth and inhibit nuclease activity (IsoCode, Schleicher and Schuell, Keene, NH). The cards are well-suited for tr...
متن کاملNeonatal buccal cell collection for DNA analysis.
It is considered undesirable to take blood from an infant as a means of obtaining DNA for research purposes. Sufficient DNA for direct gene analysis can be obtained from adult buccal epithelial cells, 1 but there is no evidence that neonatal buccal epithelial cells can similarly be used. We have carried out a preliminary study, approved by the Riverside Research Ethics Committee, to determine t...
متن کاملCollection of genomic DNA by buccal swabs for polymerase chain reaction-based biomarker assays.
Studies in molecular and genetic epidemiology require a high-throughput, low cost, and reliable means of genomic DNA collection. Buccal (cheek) swabs have been proposed as a means of achieving these goals, but there is little information about the practical application of this approach. From January 1995 to December 1997, we processed 995 buccal swabs for use in polymerase chain reaction (PCR)-...
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ژورنال
عنوان ژورنال: Cancer Epidemiology Biomarkers & Prevention
سال: 2006
ISSN: 1055-9965,1538-7755
DOI: 10.1158/1055-9965.epi-05-0753